Solid Lipid Nanoparticles of Clobetasol for Psoriasis

Solid Lipid Nanoparticles of Clobetasol for Psoriasis Formulation and Evaluation of Solid Lipid Nanoparticles of Clobetasol for Topical Treatment of Psoriasis CHAPTER 4 Methodology 4. METHODOLOGY MATERIALS USED: Table 5: LIST OF CHEMICALS USED WITH SUPPLIERS Table 6: LIST OF EUIPMENTS USED WITH SUPPLIERS Physicochemical study on the drug: Melting point determination Melting point is the temperature at which the pure liquid and solid exist in equilibrium. In practice it is taken as equilibrium mixture at an external pressure of 1 atmosphere; this is sometimes known as normal melting point. The Thiel’s tube method of melting point determination liquid paraffin was used in present study. UV spectrum UV scanning was done for pure drug from 200-400 nm in the dilution medium of methanol and in the dilution medium of phosphate buffer pH 7.4 Standard calibration curves of Clobetasol: Reagents Methanol Phosphate Buffer pH 7.4 114,115 Medium- Methanol  116 50 mg accurately weighed CP was dissolved in the methanol and volume was made up to 50ml with methanol [Stock 1]. From stock 1, different dilutions were prepared in the concentration range of 5, 10, 15, 20 and 25 µ g/ml using methanol as dilution medium. The absorbance of these solutions was measured against blank as methanol in UV spectrophotometer at 240 nm. Medium- Phosphate buffer pH 7.4 solution 50 mg accurately weighed CP was dissolved in the methanol and volume was made up to 100ml with methanol [Stock 1]. From stock 1, different dilutions were prepared in the concentration range of 5, 10, 15, 20, 25, 30 µ g/ml using Phosphate buffer pH 7.4 solution. The absorbance of these solutions was measured against Phosphate buffer pH 7.4 solution as blank in UV spectrophotometer at 239 nm. Compatibility studies of drug and polymers: FTIR spectra of pure clobetasol, physical mixtures, and SLN formulations are carried out to determine if there was any interaction between the drug and the other formulation components117. Since IR is related to covalent bonds, the spectra can provide detailed information about the structure of molecular compounds. In order to establish this  point, comparisons were made between the spectrum of the substances and pure compound. The Preformulation study was carried out prior to the development of the dosage forms. The compatibility of the drug and the excipients used were determined by IR spectrometer Shimadzu 8400 series. The spectra of formulations were compared with that of pure drug in order to ascertain for any possible interaction between polymer and drug. Preliminary studies: Initially, SLN was prepared by solvent injection method by using clobetasol (drug), carnauba wax and beeswax as lipid phase, cetyl alcohol and lecithin soya as surfactants. Tween 20 as co surfactant phase and finally distilled water to make up the volume. Where in this method, both liquid phase and lipid phase were heated to 70-75oC. When lipid phase was heated to desired temperature drug was dispersed in it and added to aqueous phase under the magnetic stirrer for 30mins. The lipid phase is added in to aquoes phase by drop wise using syringe. Thus SLNs were formed due to rapid crystallization of oil droplets and precipitation. This respective formulation design is shown in table 7. But the formed SLN was not dried and unstable. Therefore lipid extrusion method was used to prepare various formulations with different concentrations of lipid in an effort to optimize the formulations for the particle size ranging in nano scale. Table 7: Formulation design for solvent injection method The lipid extrusion method was adopted to prepare SLN. At 25mg of CP is kept constant in all formulation. Initially, the drug with different lipids tried with different concentration constant speed. The SLN were evaluated for particle size. The composition of formulation is shown in table. Formulation design:11,17,18 Procedure for preparation of SLN by lipid extrusion technique: Lipid extrusion is carried out at temperatures above the melting point of the lipid and is similar to the homogenization of an emulsion. A pre-emulsion of the drug loaded lipid melt and the aqueous emulsifier phase (same temperature) is obtained by high-shear mixing device (like Polytron PT 1600E homogenizer). High pressure homogenization of the pre-emulsion is done above the lipid melting point. Usually, lower particle sizes are obtained at higher processing temperatures because of lowered viscosity of the lipid phase. Fig 11: Schematic representation of SLN preparation by lipid extrusion Table 8: Formulation design for lipid extrusion method Table 9: Formulation design with stirring speed and duration of rotation using Polytron pt 1600E for Formulation F EVALUTION OF SLN: Physical Evaluations: Visual appearance pH: The pH of SLN formulations were measured using pH paper. Rheological studies Rheological properties (study of deformation and flow of matter) are required in various pharmaceutical areas. It helps to monitor the effect of vehicles consistency on release of drug from the preparations and subsequent percutaneous absorption. Also it is important from the manufacturing point of view. Viscosity measurements were carried out using a Brookfield viscometer (T – bar spindle). The formulation of SLN was kept in 100ml beaker and dial readings was noted at 3, 5, 6, 10, 12, 20, 30, 50 and 60 rpm. The speed was then successively lowered and the corresponding dial readings were noted. Particle Size Analysis118: The particle size should be less than 1000 nm in nanoparticles. It can be analyzed by using Malvern particle size analyzer. Particles in the size range of colloids display constant random thermal motion which is known as Brownian motion. This motion causes the intensity of light scattered by the particles to vary with time. The larger the particle slower their motion and hence the smaller the variation in intensity of light scattered. Photon correlation spectroscopy uses the rate of change in the intensity to determine the size distribution of particles. The zetasizer has a correlator with 64 channels. Each of this channel measures changes in light fluctuation over a defined time span. The time span is known as the sample time or delay time the correlator measures the light intensity by counting photons. For a very short time period the changes in light intensities will be very small as the particles had very little time to move. The position of the particles can be statistically defined as being highly correlated, Contrast to this with a long sample time, particles will have moved randomly from their initial positions. Therefore the particles can be described statistically as not being correlated. Zeta potential measurement Zeta potential of the SLNs were measured by malveren zetasizer. The zetasizer mainly consist of laser which is used to provide a light source to illuminate the particles within the sample. For zetapotential measurements this light splits to provide an incident and reference beam. The incident laser beam passes through the centre of the sample cell, and the scattered light at an angle of about 130 is detected. when an electric field is applied to the cell, any particles moving through the measurement volume will cause the intensity of light detected to fluctuate with a frequency proportional to the particle speed and this information is passed to the digital signal possessor and then to a computer. Zetasizer software produces a frequency spectrum from which the electrophoretic mobility hence the zeta potential  is calculated. Scanning Electron Microscopy (SEM): Surface morphology of the specimen will be determined by using a scanning electron microscope. Procedure: The samples are dried thoroughly in vaccum desicator before mounting on brass specimen studies, using double sided adhesive tape. Gold-palladium alloy of 120 °A Knees was coated on the sample sputter coating unit (Model E5 100 Polaron U.K) in Argon at ambient of 8-10 with plasma voltage about 20mA. The sputtering was done for nearly 5 minutes to obtain uniform coating on the sample to enable good quality SEM images. The SEM was operated at low accelerating voltage of about 15KV with load current about 80mA.The condenser lens position was maintained betwee 4.4-5.1. The objective lens aperture has a diameter of 240 microns and working distance WD=39mm. Drug content119: The drug equivalent to 10 mg of formulation was taken and dissolved in small quantity of methanol. Then the formulation is warmed on the water bath so that the drug present in the formulation was completely dissolved. Then the solution was filtered through Whattman filter paper in 25 ml. volumetric flask and volume was made up to the mark by methanol to give concentration of 1000 ÃŽ ¼g/ml. for Clobetasol. Then 1 ml. was pipetted out in 100 ml. volumetric flask to give concentration of 10ÃŽ ¼g/ml and then absorbance was measured at 240 nm. In-vitro release studies114,115,120: In Franz diffusion cell, 6 gm. of sample was kept in donor compartment. The entire surface of cellophane membrane was in contact with the receptor compartment containing 50 ml of phosphate buffer pH 7.4. The receptor compartment was continuously stirred using the magnetic stirrer. The temperature was maintained 35 °C. The study was carried out for 24 hrs and the sample was withdrawn at 30 minute time interval and same volume was replaced with free phosphate buffer. The content of clobetasol from withdrawn sample was measured after suitable dilution. Stability studies: Whenever a new formulation is developed, it is very essential to establish that the therapeutic activity of the drug has not undergone any change. To conform this, the selected formulations were subjected to stability studies. Generally, the observation of the rate at which the product degrades under normal room temperature requires long time. To avoid this undesirable delay, the principles of the accelerated stability studies are adopted. The International Conference of Harmonization guidelines titled â€Å"stability testing for drug substance and product† describes the stability tests requirements for drug registration applications in the European Union, Japan and United States of America. Table 10: International climatic zones and climatic conditions Table 11: General stability testing consideration Study

being there Essay -- essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  Being There is the story of Chance, a simple gardener turned American media hero. He seems to know nothing but television and gardening. His thoughts and judgments are products of television and his gardening experience. Yet through his simple mild mannered ways he unintentionally becomes the center of America’s business news. The author of Being There, Jerzy Kosinski said â€Å"To read a novel is to practice for real life. Fiction doesn’t change anybody’s life, it merely hints at the different ways of looking at oneself, at others, and at society† Since Chance was not able to read, television shows were his novels. Television was his practice for real life.   Ã‚  Ã‚  Ã‚  Ã‚  Television was like a mirror for Chance. Chance felt, â€Å"by changing the channel he could change himself† He was able to imagine himself to be people on television. I believe this is why Chance was able to put on the appearance he had even if it was unintentional. His appeal and gestures were all products of TV’s influence. With the help of the old man’s suit, Chance passed for a wealthy business man without even trying or knowing.   Ã‚  Ã‚  Ã‚  Ã‚  Having no contact with the outside world growing up, television was Chance’s only hint of what society was like. By flipping through the channels, Chance noticed the different ways people would interact with each other. Television provided him with the hints of different ways of looking at people and society. ...

Research Qualitative Review

This paper will critique the qualitative study written by Peiters, et al. (2011), which looks at the journey and barriers older women diagnosed with breast cancer must hurdle to receive treatment. This research was to reveal the complexities of breast cancer diagnosis, treatment and life in general for women greater than seventy-years old. To achieve this task, Peiters, et al. , (2011) implemented the constructivist grounded theory. The data analysis approach appropriately utilized for this research was constructivist grounded theory. This theory allows for self reflection during data gathering and analysis, which divulges any influential prejudices that may arise from both researcher and study subject. Influencing this theory is symbolic interactionism which emphasizes ones’ ultimate ambition and the lifelong social synergy that transpires to achieve it (Peiters, et al. , 2011). The recruitment of participants was obtained by posting flyers in public areas in Southern California and two bordering states. The locations targeted were oncology departments, cancer support agencies, oncologist offices, churches, senior community centers, and retirement centers. Newspaper advertisements, as well were used to elicit participation. Purposive and snowballing techniques were utilized. Screening for eligibility, clearly defined, was done via telephone for inclusions which ended with eighteen women all over the age of seventy, completed treatment for treatment for breast cancer within the last three to fifteen months. Informed consent was obtained and interviews took place mainly in homes or apartments lasting up to two and a half hours. One limitation of the study was that second interviews stopped at only ten women and may have excluded valuable new incite to the study, although researchers claim that second interviews revealed no new data, hence implying data saturation. Adequate thick description was given of the participants, setting and study processes to ensure transferability (Polit & Beck, 2012). Interviews were guided by a questionnaire and responses were tape recorded, followed by verbatim transcription. Examples of interview questions were provided for the reader to convey effectiveness of the process (Polit & Beck, 2012). Other helpful tables provided were the sociodemographic and clinical characteristics. Category schemes revealed are three barriers that theses breast cancer survivors faced, which were lack of information, preexisting co-morbidities and multiple health care appointments. These schemes prove logical for the study as they helped elaborate the purpose of the research. Multiple passages from the participants were extracted to give evidence of well interpreted data and emotional state of the participants. Data analysis was systematic initial coding, followed by focused coding using ATLAS. ti, version 6. to magnify reoccurring data. To create a audit trail, memos, field notes and diagrams were written. Two researchers, one of them being the principle investigator and the other well versed in grounded theory analysis, were responsible for coding and analyzing the data. In nursing the level of evidence provides assurance for the methods that were utilized for the study (Polit & Beck, 2012). This research would qualify as level VI, given that it is a single descriptive, qualitative, physiologic study specific to women aged seventy or older and their journey through life with breast cancer. The goals of this research are affirmed by the extensive detailed interviews of eighteen older women and their struggles with breast cancer. Each participant plummeted through barricades to recieveing health care. The importance of an oncology nurse navigator emerged as a valuable role to help clarify the process of obtaining services for healthcare. Meticulous care was taken in the transcription of taped interviews and explicit excerpts were bestowed to the reader to ensure trustworthiness.

Walk Into School And Class For The First Time Since High...

I’m about to walk into school and go to class for the first time since high school. It’s been fifteen years and I never thought that I would make it back to school. I mean I had hope to go to school again just didn’t ever really think about it. When life hits hard, people just don’t have as much time to do the things they grew up thinking they would do. Stop and smell the roses, that kind of thing. Well that’s going to change about my life, I’m going to be the guy that went back to school. I dropped out of school when I was seventeen. At the time I wasn’t living with my parents and wasn’t in Tennessee where I grew up. It was new and exciting to me, living in a big city, working, and doing my own thing in Dallas, Texas. Five years go by and I had a good job and started to work a second job for the extra money. I started hanging out with college kids thinking to myself that this is what I need to be doing with my life, going back to school, getting a good job, and being able to say to my kids that I had my own success story. I set up an appointment to take the GED and thought this is going to go great. Thought I would have time to study and get a good score. Then I got tired of studying and juggling my time at home with the kids and wife. I went to take the test thinking that I remember most of what I learned in high school. I flew through the test and the teachers there were asking me if I was sure that I didn’t need more time. They told me good luck as I was leaving becauseShow MoreRelatedEssay On A Whole New World890 Words   |  4 Pagesbed on August 10th. When I realized what time it was my mind blew into a million pieces. I was finally going to be in high school, and I couldn’t believe the first day of my high school experience had finally come. 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